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hcr hifi probe hybridization buffer  (Molecular Instruments)

 
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    Structured Review

    Molecular Instruments hcr hifi probe hybridization buffer
    (A,B-A””,B””) <t>Hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).
    Hcr Hifi Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcr+hifi+probe+hybridization+buffer/buffer+probe+wash/pmc12747729-433-4-9
    Average 86 stars, based on 1 article reviews
    hcr hifi probe hybridization buffer - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "PRDM paralogs are required for Meckel’s cartilage formation during mandibular bone development"

    Article Title: PRDM paralogs are required for Meckel’s cartilage formation during mandibular bone development

    Journal: Developmental biology

    doi: 10.1016/j.ydbio.2025.12.002

    (A,B-A””,B””) Hybridization chain reaction (HCR) was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).
    Figure Legend Snippet: (A,B-A””,B””) Hybridization chain reaction (HCR) was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).

    Techniques Used: Expressing, Hybridization, Gene Expression, Quantitative RT-PCR, Isolation, Control, Two Tailed Test

    Related Articles

    Hybridization:

    Article Title: PRDM paralogs are required for Meckel’s cartilage formation during mandibular bone development
    Article Snippet: .. Sections were pre-hybridized in HCR HiFi Probe Hybridization Buffer (Molecular Instruments) for 10 minutes at 37°C in a humidified chamber. .. Mandibular processes were lysed in TRIzol LS (Invitrogen/Life Technologies) and total RNA was isolated from these samples using a chloroform extraction.



    Similar Products

    hcr hifi probe hybridization buffer  (Molecular Instruments)
    86
    Molecular Instruments hcr hifi probe hybridization buffer
    (A,B-A””,B””) <t>Hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).
    Hcr Hifi Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcr+hifi+probe+hybridization+buffer/buffer+probe+wash/pmc12747729-433-4-9
    Average 86 stars, based on 1 article reviews
    hcr hifi probe hybridization buffer - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    (A,B-A””,B””) Hybridization chain reaction (HCR) was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).

    Journal: Developmental biology

    Article Title: PRDM paralogs are required for Meckel’s cartilage formation during mandibular bone development

    doi: 10.1016/j.ydbio.2025.12.002

    Figure Lengend Snippet: (A,B-A””,B””) Hybridization chain reaction (HCR) was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).

    Article Snippet: Sections were pre-hybridized in HCR HiFi Probe Hybridization Buffer (Molecular Instruments) for 10 minutes at 37°C in a humidified chamber.

    Techniques: Expressing, Hybridization, Gene Expression, Quantitative RT-PCR, Isolation, Control, Two Tailed Test